Ruprecht-Karls-Universität Heidelberg
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Greb0119 - Scientist (f/m) / PhD position
Project no:
Greb0119

Project leader:

Project supervisor:
Greb, Thomas
Application deadline:
28. Feb 2019
Start of PhD project:
1. May 2019

Project description:

Title:
Analysis of cell fate decisions during lateral plant growth
Summary:
Radial growth of plant shoots and roots is based on the activity of a group of stem cells, the cambium, and essential for generating large plant bodies. Thereby, it substantially contributes to the dominance of seed plants in terrestrial ecosystems, to wood formation and thus to carbon immobilization. In addition, the process represents a striking example for a constant remodelling of adult body structures and for stem cell regulation. Thus, lateral growth provides excellent opportunities for addressing the fundamental question of how cellular properties are integrated and adapted during the growth of adult organisms. Depending on the interest of the student, we offer the opportunity to investigate cell fate regulation in the context of cambium activity with a molecular, biochemical or computational angle.
References:
Shi D, Lebovka I, López-Salmerón V, Sanchez P, Greb T (2019) Bifacial cambium stem cells generate xylem and phloem during radial plant growth. Development

Brackmann K, Qi J, Gebert M, Jouannet V, Schlamp T, Grünwald K, Wallner ES, Novikova DD, Levitsky VG, Agusti J, Sanchez P, Lohmann JU, Greb T (2018) Spatial specificity of auxin responses coordinates wood formation. Nat Commun 9(1):875.

Wallner ES, López-Salmerón V, Belevich I, Poschet G, Jung I, Grünwald K, Sevilem I, Jokitalo E, Hell R, Helariutta Y, Agustí J, Lebovka I, Greb T (2017) Strigolactone and karrikin-independent SMXL proteins are central regulators of phloem formation. Curr Biol 27(8):1241–1247.

Tonn N, Greb T (2017) Radial plant growth. Curr Biol 27(17):R878–R882.

Greb T, Lohmann JU (2016) Plant Stem Cells. Curr Biol 26(17):R816-R821.
Methods that will be used:
Transcriptomics
Computational Modelling
Biochemistry
Histology
Confocal microscopy
Cooperation partners:
Jan Lohmann (Heidelberg University), Sebastian Wolf (Heidelberg University)
Personal qualifications:
The ideal candidate should be willing to learn and establish novel technologies and be interested in quantitative aspects of developmental processes and in evolution. A background in molecular biology, imaging, image analysis or computational modelling would be desirable. He/she should also be able to work independently as well as in a team, be well organised and prepared to work in an international environment. Good interpersonal communication skills are essential.
Keywords:
Stem cell biology, cambium, evolution, cell fate regulation