Ruprecht-Karls-Universität Heidelberg
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Schiebel0219 - Scientist (f/m) / PhD position
Project no:
Schiebel0219

Project leader:

Project supervisor:
Schiebel, Elmar
Application deadline:
31. Jul 2019
Start of PhD project:
1. Sep 2019

Project description:

Title:
Microtubules – building them at the right time, at the right place
Summary:
Microtubules are highly dynamic polymers with essential functions in chromosome segregation in mitosis and meiosis, intracellular organization, cell motility and neurogenesis. In addition, they are targets for drugs that are used in cancer therapy (Paclitaxel and Vinca alkaloids). Microtubule malfunction is associated with cancer, infertility and neurological diseases.

Using gamma-tubulin complexes, cells have developed mechanisms for the assembly of microtubules from tubulin subunits. The activity of this basic assembly machinery is modulated in time and space by accessory factors, for example microtubule polymerases and activators such as the microcephaly protein CDK5RAP2. In this project we aim to understand when and where these co-factors come in place and how their functions are changed in cancer and adapted in specific cell types, for example neurons.

We offer:
The PhD position is funded for 3 years with starting date based on mutual agreement (as soon as possible). The remuneration is based on TV-L.

Interested?
Please send applications to E. Schiebel: schiebel.elmar@zmbh.uni-heidelberg.de

References:
Gunzelmann J, Ruthnick D, Lin TC, Zhang W, Neuner A, Jakle U, Schiebel E (2018) The microtubule polymerase Stu2 promotes oligomerization of the gamma-TuSC for cytoplasmic microtubule nucleation. Elife 7: e39932

Lin TC, Neuner A, Flemming D, Liu P, Chinen T, Jakle U, Arkowitz R, Schiebel E (2016) MOZART1 and gamma-tubulin complex receptors are both required to turn gamma-TuSC into an active microtubule nucleation template. J Cell Biol 215: 823-840

Lin TC, Neuner A, Schiebel E (2015) Targeting of gamma-tubulin complexes to microtubule organizing centers: conservation and divergence. Trends Cell Biol 25: 296-307

Lin TC, Neuner A, Schlosser YT, Scharf AN, Weber L, Schiebel E (2014) Cell-cycle dependent phosphorylation of yeast pericentrin regulates gamma-TuSC-mediated microtubule nucleation. Elife 3: e02208

Gombos L, Neuner A, Berynskyy M, Fava LL, Wade RC, Sachse C, Schiebel E (2013) GTP regulates the microtubule nucleation activity of gamma-tubulin. Nat Cell Biol 15: 1317-27Erlemann S, Neuner A, Gombos L, Gibeaux R, Antony C, Schiebel E (2012) An extended γ-tubulin ring functions as a stable platform in microtubule nucleation. J Cell Biol 197: 59-74

Knop M, Schiebel E (1998) Receptors detemrine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation. EMBO J 17:3952-3967
Methods that will be used:
The PhD student will be using a broad range of techniques such as biochemical approaches, super resolution microscopy (STED and SIM), cryo-electron microscopy, CRISPR/Cas9 technology for genomic knockins and knockouts and live cell imaging to study microtubule assembly.
Cooperation partners:
Personal qualifications:
Highly motivated PhD students with a background in biochemistry, cell biology or molecular biology should apply. Successful candidates will be part of an international team of PhD students and postdocs that works at the forefront of scientific research.
Keywords: