Ruprecht-Karls-Universität Heidelberg
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Brügger, Britta , Prof. Dr. (Project leader)
Biochemistry Center Heidelberg (BZH)
Complete address:
Heidelberg University
Biochemistry Center (BZH)
Im Neuenheimer Feld 328, R308
69120 Heidelberg
+49 6221-54 5326
+49 6221-54 4366

Fields of interest:

We are interested in identifying and characterising specific protein-lipid interactions within membranes. To study such interactions we employ complementary approaches including cellular, in vitro and in silico systems. With a combination of chemical biology and mass-spectrometry based lipidomics we have recently discovered a specific interaction of a type I membrane protein with N-stearoyl-sphingomyelin. Here, lipid-binding modulates protein activity by facilitating protein dimerisation. Using a bioinformatics approach we defined a novel sphingolipid-binding motif within the transmembrane domain of a number of mammalian membrane proteins. We now aim at understanding structure-function-relationships of selected candidate proteins.
Lipidomics, Protein-Lipid-Interactions, Sphingolipids, Membrane proteins, Vesicular Transport

Curriculum Vitae:

Research Fellow at Heidelberg University Biochemistry Center

Postdoctoral Research Fellow with Prof. Dr. Felix Wieland at Heidelberg University Biochemistry Center, Germany

Postdoctoral Research Fellow with Prof. Dr. James E. Rothman at Cellular Biochemistry and Biophysics Program, Sloan-Kettering Institute, New York, USA

Ph.D. thesis with Prof. Dr. Felix Wieland at Institute for Biochemistry, Ruprecht Karls University, Heidelberg, Germany

Study of Biochemistry at Frankfurt University, Germany

Selected publications:

Sphingolipids as modulators of membrane proteins (2013) Ernst MA, Brügger B BBA MCBL, doi:pii: S1388-1981(13)00239-4

In vivo profiling and visualization of cellular protein-lipid interactions using bifunctional fatty acids. (2013) Haberkant P, Raijmakers R, Wildwater M, Sachsenheimer T, Brügger B, Maeda K, Houweling M, Gavin AC, Schultz C, van Meer G, Heck AJ, Holthuis JC. Angew Chem Int Ed Engl 52(14):4033-8-

Differential transport of Influenza A neuraminidase signal anchor peptides to the plasma membrane. (2013) Ernst AM, Zacherl S, Herrmann A, Hacke M, Nickel W, Wieland FT, Brügger B. FEBS Lett 587(9):1411-7.

Comparative lipidomics analysis of HIV-1 particles and their producer cell membrane in different cell lines. (2013) Lorizate M, Sachsenheimer T, Glass B, Habermann A, Gerl MJ, Kräusslich HG, Brügger B. Cell Microbiol 15(2):292-304.

Quantitative analysis of cellular lipids by nano-electrospray ionisation mass spectrometry. (2013) Özbalci C, Sachsenheimer T, Brügger B Methods Mol Biol, 1033:3-20

The Structures of COPI-Coated Vesicles Reveal Alternate Coatomer Conformations and Interactions. (2012) Faini M, Prinz S, Beck R, Schorb M, Riches JD, Bacia K, Brügger B, Wieland FT, Briggs JA. Science 336(6087):1451-4.

Molecular recognition of a single sphingolipid species by a protein's transmembrane domain (2012) Contreras FX, Ernst AM, Haberkant P, Björkholm P, Lindahl E, Gönen B, Tischer C, Elofsson A, von Heijne G, Thiele C, Pepperkok R, Wieland F, Brügger B. Nature 481(7382):525-9

Biochemical and morphological properties of hepatitis C virus particles and determination of their lipidome. (2011) Merz A, Long G, Hiet MS, Brügger B, Chlanda P, Andre P, Wieland F, Krijnse-Locker J, Bartenschlager R. J Biol Chem. 286(4):3018-32.

A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4. (2010) Osman C, Haag M, Wieland FT, Brügger B, Langer T. EMBO J 29(12):1976-87.

Genetic evidence for functional interaction of the Escherichia coli signal recognition particle receptor with acidic lipids in vivo. (2010) Erez E, Stjepanovic G, Zelazny AM, Brügger B, Sinning I, Bibi E. J Biol Chem. 285(52):40508-14.

Ceramide triggers budding of exosome vesicles into multivesicular endosomes. (2008) Trajkovic K, Hsu C, Chiantia S, Rajendran L, Wenzel D, Wieland F, Schwille P, Brügger B, Simons M. Science 319(5867):1244-7.

Protein-sphingolipid interactions within cellular membranes. (2008) Haberkant P, Schmitt O, Contreras FX, Thiele C, Hanada K, Sprong H, Reinhard C, Wieland FT, Brügger B. J Lipid Res 49(1):251-62.

Molecular anatomy of a trafficking organelle. (2006) Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, Rammner B, Gräter F, Hub JS, De Groot BL, Mieskes G, Moriyama Y, Klingauf J, Grubmüller H, Heuser J, Wieland F, Jahn R. Cell 127(4):831-46.

The HIV lipidome: a raft with an unusual composition. (2006) Brügger B, Glass B, Haberkant P, Leibrecht I, Wieland FT, Kräusslich HG. PNAS 103(8):2641-6.

High cholesterol level is essential for myelin membrane growth. (2005) Saher G, Brügger B, Lappe-Siefke C, Möbius W, Tozawa R, Wehr MC, Wieland F, Ishibashi S, Nave KA. Nat Neurosci 8(4):468-75.

Evidence for segregation of sphingomyelin and cholesterol during formation of COPI-coated vesicles. (2000) Brügger B, Sandhoff R, Wegehingel S, Gorgas K, Malsam J, Helms JB, Lehmann WD, Nickel W, Wieland FT. J Cell Biol 151(3):507-18.

Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry. (1997) Brügger B, Erben G, Sandhoff R, Wieland FT, Lehmann WD. PNAS 94(6):2339-44.