Ruprecht-Karls-Universität Heidelberg
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Boutros0117 - Scientist (f/m) / PhD position
Project no:
Boutros0117

Project leader:

Project supervisor:
Boutros, Michael
Application deadline:
10. Jul 2017
Start of PhD project:
1. Aug 2017

Project description:

Title:
Switches in Wnt trafficking and signaling
Summary:
Signaling molecules control the development in all multicellular organisms and are frequently deregulated in cancer. This PhD project will focus on the systematic analysis of master regulators of intra- and extracellular trafficking of signaling proteins, which play crucial roles in tissue patterning, stem cell maintenance and the regulation of cell fate decisions. Work will be mainly conducted in the Drosophila model system, which allows to study signaling at the organismal, tissue, cellular and molecular level and offers a large set of sophisticated genetic tools. As part of the PhD project, you will generate tagged Drosophila lines to create specific molecular switches using state of the art genome engineering techniques and analyze them by time-resolved high-resolution imaging of intact tissues. You will also employ novel transgenic tools currently developed in our lab to systematically knock-out genes controlling membrane trafficking in a tissue-specific fashion and analyze the intra- and extracellular trafficking of Wnt proteins.
References:
Bartscherer, K., Pelte, N., Ingelfinger, D., Boutros, M. (2006). Secretion of Wnt ligands requires Evi, a conserved transmembrane protein. Cell 125:523-33.

Gross, J., Chaudhary, V., Bartscherer, K., Boutros, M. (2012). Active Wnt
proteins are secreted on exosomes. Nature Cell Biology 14:1036-45.

Demir, K., Kirsch, N., Beretta, C.A., Erdmann, G., Ingelfinger, D., Moro, E., Argenton, F., Carl, M., Niehrs, C., Boutros, M. (2013). RAB8B is required for activity and caveolar endocytosis of LRP6. Cell Reports 4:1224-34.

Please also visit our Website: http://www.boutroslab.org
Methods that will be used:
Genome engineering; CRISPR; live and high-resolution imaging; signal transduction studies; genetics
Cooperation partners:
Personal qualifications:
Applicants should hold a diploma/masters’ degree in biology /life sciences with an excellent background in cell biology, genetics or biochemistry and have strong laboratory, analytical and communication skills. The candidate should be enthusiastic, motivated, result-driven, and a critical thinker. The application should have good organization, writing and communication skills and be able to work well both independently and in a team environment.
Keywords:
Wnt signaling, intracellular trafficking, developmental genetics, imaging, CRISPR, Drosophila