Ruprecht-Karls-Universität Heidelberg
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Breuhahn0117 - Scientist (f/m) / PhD position
Project no:
Breuhahn0117

Project leader:

Project supervisor:
Breuhahn, Kai
Application deadline:
21. Oct 2017
Start of PhD project:
1. Dec 2017

Project description:

Title:
Tumor suppressive mechanisms in liver cance
Summary:
The groups of Dr. Stephanie Rössler and Dr. Kai Breuhahn at the Institute of Pathology at Heidelberg University Hospital are seeking a highly motivated PhD student. Our laboratory is interested in the role of genetic alterations in hepatocellular carcinoma (HCC). Liver cancer is the second leading cause of cancer-related death worldwide. The high mortality rate of HCC is mainly caused by metastasis or by de novo tumor formation in the diseased liver.
The project of the successful candidate will focus on the functional characterization of tumor suppressor genes on chromosome 8p in HCC. We employ a broad range of techniques, including mouse models, molecular biology and biochemistry (protein-protein interactions), cell biology (live-cell imaging) and computational analysis to connect molecular biology and medical relevance.
We offer a friendly and productive interdisciplinary work atmosphere and state-of-the-art equipment within the newly established and DFG funded SFB “Liver cancer”. The PhD student will be part of the local PhD Program, which offers interdisciplinary practical training, scientific conferences, mentoring by a thesis committee of experts in the field, and a highly stimulating international research environment.
References:
• Weiler S, Pinna F, Wolf T, Lutz T, Geldiyev A, Sticht C, Knaub M, Thomann S, Bissinger M, Wan S, Rössler S, Becker D, Gretz N, Lang H, Bergmann F, Ustiyan V, Kalin TV, Singer S, Lee JS, Marquardt JU, Schirmacher P, Kalinichenko VV, Breuhahn K (2017) Induction of Chromosome Instability by Activation of Yes-Associated Protein and Forkhead Box M1 in Liver Cancer. Gastroenterology 152(8):2037-2051

• Ploeger C, Waldburger N, Fraas A, Goeppert B, Pusch S, Breuhahn K, Wang XW, Schirmacher P, Roessler S (2016) Chromosome 8p tumor suppressor genes SH2D4A and SORBS3 cooperate to inhibit interleukin-6 signaling in hepatocellular carcinoma. Hepatology 64(3):828-42

• Samarin J, Laketa V, Malz M, Roessler S, Stein I, Horwitz E, Singer S, Dimou E, Cigliano A, Bissinger M, Falk CS, Chen X, Dooley S, Pikarsky E, Calvisi DF, Schultz C, Schirmacher P, Breuhahn K (2016) PI3K/AKT/mTOR-dependent stabilization of oncogenic far-upstream element binding proteins in hepatocellular carcinoma cells. Hepatology 63(3):813-26

• Roessler S, Long EL, Budhu A, Chen Y, Zhao X, Ji J, Walker R, Jia HL, Ye QH, Qin LX, Tang ZY, He P, Hunter KW, Thorgeirsson SS, Meltzer PS, Wang XW (2012) Integrative genomic identification of genes on 8p associated with hepatocellular carcinoma progression and patient survival. Gastroenterology 142(4): 957-66

• Roessler S, Jia HL, Budhu A, Forgues M, Ye QH, Lee JS, Thorgeirsson SS, Sun Z, Tang ZY, Qin LX, Wang XW (2010) A unique metastasis gene signature enables prediction of tumor relapse in early-stage hepatocellular carcinoma patients. Cancer Res 70(24):10202-12
Methods that will be used:
• Culture and transfection of human cell line
• Live-cell imaging
• Molecular biology techniques, i.e. Western blot, qPCR
• Functional assays, i.e. cell viability, migration, invasion
• Murine liver cancer models
Cooperation partners:
Jane McKeating (Oxford, UK)
Xin Wei Wang (Bethesda, MD, USA)
Personal qualifications:
We are looking for a highly motivated PhD student with a background in molecular biology, biochemistry or cell biology. Applicants must have a MSc degree or equivalent in Biology, Biochemistry or related fields. Expertise in tissue culture, microscopy and molecular biology is desired. We expect enthusiasm for science and strong work ethics.
Applications (cover letter, statement of research interests, CV, qualifications and contact information of two to three referees) should be sent in one pdf-document to Dr. Stephanie Rössler: Stephanie.Roessler@med.uni-heidelberg.de
Possible start of employment: immediately
Contract: 3 year fixed-term contract with possibility of extension
Salary: E13 TV-L, 65%
Contact person: Dr. Stephanie Rössler/Dr. Kai Breuhahn
Email: Stephanie.Roessler@med.uni-heidelberg.de
Webpage: https://www.klinikum.uni-heidelberg.de/index.php?id=138014&L=1
Keywords:
Tumor biology, liver cancer, tumor suppressor genes, molecular biology