Ruprecht-Karls-Universität Heidelberg
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Herten0118 - Scientist (f/m) / PhD position
Project no:

Project leader:

Project supervisor:
Herten, Dirk-Peter
Application deadline:
30. Apr 2018
Start of PhD project:
1. Jun 2018

Project description:

Stoichiometric protein labelling for quantitative fluorescence microscopy
Counting of protein numbers and their interaction kinetics is gaining more and more importance in the area of quantitative (cell) biology. We have developed a method for counting the number of protein copies in cellular structures in situ based on single-molecule fluorescence microscopy. Using a single-photon sensitive confocal microscope we measure the photon statistics in microscopic samples and determine copy numbers of fluorescently labelled proteins present in isolated protein clusters.
We are looking for a PhD student in biology, biochemistry or chemistry to establish procedures in molecular and cell biology for quantitative and stoichiometric labelling of proteins thereby achieving a direct relation between the number of fluorophores determined and the number of proteins present in the cell. To this end, we aim at establishing gene editing, i.e. CRISPR/CAS, in our lab in collaboration with different partners in biology.
K. S. Grußmayer, D.-P. Herten "Time-resolved Molecule Counting by Photon Statistics Across the Visible Spectrum" (2017) PCCP 19, 8962–8969

K. S. Grußmayer, A. Kurz, D.-P. Herten "Single-molecule studies on the label number distribution of fluorescent markers" (2014) ChemPhysChem 15, 4, 734–742.
Methods that will be used:
Cloning, cell culturing, fluorescence labelling, single-molecule fluorescence microscopy
Cooperation partners:
Prof. Oliver Fackler (Virology, Heidelberg Univ.)
Personal qualifications:
Interested candidates should hold a degree in biology, biochemistry, or molecular biotechnology. Experience in molecular and cell biology is essential. We expect excellent oral and written communication skills; strong team working skills; an ability to take initiative to solve problems; strong motivation in driving projects forward; and a significant critical thinking capability. Candidates with previous experience of in vivo imaging or biomedical optics, for example during a Masters or Diploma thesis, are particularly encouraged to apply.
Quantitative microscopy, single-molecule fluorescence microscopy, protein tags, CRISPR/CAS