Ruprecht-Karls-Universität Heidelberg
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Herten0118 - Scientist (f/m) / PhD position
Project no:
Herten0118

Project leader:

Project supervisor:
Herten, Dirk-Peter
Application deadline:
30. Apr 2018
Start of PhD project:
1. Jun 2018

Project description:

Title:
Stoichiometric protein labelling for quantitative fluorescence microscopy
Summary:
Counting of protein numbers and their interaction kinetics is gaining more and more importance in the area of quantitative (cell) biology. We have developed a method for counting the number of protein copies in cellular structures in situ based on single-molecule fluorescence microscopy. Using a single-photon sensitive confocal microscope we measure the photon statistics in microscopic samples and determine copy numbers of fluorescently labelled proteins present in isolated protein clusters.
We are looking for a PhD student in biology, biochemistry or chemistry to establish procedures in molecular and cell biology for quantitative and stoichiometric labelling of proteins thereby achieving a direct relation between the number of fluorophores determined and the number of proteins present in the cell. To this end, we aim at establishing gene editing, i.e. CRISPR/CAS, in our lab in collaboration with different partners in biology.
References:
K. S. Grußmayer, D.-P. Herten "Time-resolved Molecule Counting by Photon Statistics Across the Visible Spectrum" (2017) PCCP 19, 8962–8969

K. S. Grußmayer, A. Kurz, D.-P. Herten "Single-molecule studies on the label number distribution of fluorescent markers" (2014) ChemPhysChem 15, 4, 734–742.
Methods that will be used:
Cloning, cell culturing, fluorescence labelling, single-molecule fluorescence microscopy
Cooperation partners:
Prof. Oliver Fackler (Virology, Heidelberg Univ.)
Personal qualifications:
Interested candidates should hold a degree in biology, biochemistry, or molecular biotechnology. Experience in molecular and cell biology is essential. We expect excellent oral and written communication skills; strong team working skills; an ability to take initiative to solve problems; strong motivation in driving projects forward; and a significant critical thinking capability. Candidates with previous experience of in vivo imaging or biomedical optics, for example during a Masters or Diploma thesis, are particularly encouraged to apply.
Keywords:
Quantitative microscopy, single-molecule fluorescence microscopy, protein tags, CRISPR/CAS