Ruprecht-Karls-Universität Heidelberg
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Thi0119 - Scientist (f/m) / PhD position
Project no:
Thi0119

Project leader:

Project supervisor:
Thi, Viet Loan Dao
Application deadline:
5. Oct 2019
Start of PhD project:
1. Dec 2019

Project description:

Title:
Studying the innate immune response of hepatitis E virus infection in human pluripotent stem cell-derived hepatocyte-like systems
Summary:
Hepatitis E virus (HEV) is the major cause of acute hepatitis in the world. Our lab uses human embryonic or induced pluripotent stem cell technology to study HEV life cycle and HEV-host interactions. The succesful applicant will combine these stem cell systems with genetic, biochemical, and imaging approaches to characterize the cellular HEV-induced innate immune response, the first line of defense against invading pathogens.
More information about the lab at daothilab.com. For further questions, please contact: VietLoan.DaoThi@med.uni-heidelberg.de
References:
1. Wu X*, Dao Thi VL*, Liu P, Takacs CN, Xiang K, Andrus L, Gouttenoire J, Moradpour D, and Rice CM (2018). Pan-Genotype Hepatitis E Virus Replication in Stem Cell-Derived Hepatocellular Systems. Gastroenterology. 154(3):663-674.e7

2. Wu X, Dao Thi VL, Huang Y, Billerbeck E, Saha D, Hoffmann HH, Wang Y, Vale Silva LA, Sarbanes S, Sun T, Andrus L, Quirk C, MacDonald MR, Schneider WM, An X, Rosenberg BR, and Rice CM (2018). Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 172(3):423-438.e25

Methods that will be used:
Cooperation partners:
A wide range of molecular and cell biological techniques with emphasis on live cell imaging using confocal microscopy; human tissue culture including embryonic and induced pluripotent stem cells and their differentiation to various cell types, mainly hepatocytes; 3D and complex co-culture systems; infection studies with human viruses in BSL2 and potentially BSL3 laboratories.
Personal qualifications:
We are looking for a highly motivated, interactive and team-oriented candidate with a background and interest in molecular biology and virology. Applicants should hold a Master’s degree in Biomedical or Molecular Sciences, Biochemistry, Biology or related fields. Expertise in cell imaging and confocal microscopy is advantageous.
Keywords:
Molecular virology, stem cells, innate immunity