Ruprecht-Karls-Universität Heidelberg
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Enk0120 - Scientist (f/m) / PhD position
Project no:
Enk0120

Project leader:

Project supervisor:
Enk, Alexander
Hadaschik, Eva
Application deadline:
15. Aug 2020
Start of PhD project:
1. Sep 2020

Project description:

Title:
Role of Regulatory T cells in B cell-mediated Autoimmune Skin Disease
Summary:
Autoimmune bullous dermatoses (AIBD) are severe autoantibody-mediated diseases of skin and mucous membranes. Present autoantibodies against structural proteins of desmosomes and hemidesmosomes lead to the recruitment and activation of immune cells which results in the formation of blisters between epidermis and dermis. Regarding the specificity of each autoantibody we can distinguish between different subforms of AIBDs, like bullous pemphigoid and pemphigus. So far, the exact pathomechanism of certain autoantibodies in AIBDs is not well understood.
We work in our group with a mouse model called Scurfy, where the affected mice spontaneously develop skin blistered caused by different autoantibodies with different specificities. The aim of this project is the investigation of the pathomechanism of different autoreactive autoantibodies in the setting of AIBD. Furthermore, the role of T follicular helper cells (Tfh) and follicular regulatory T cells (Tfr) for the induction of autoantibodies will be investigated by using different established mouse models.
References:
Treg deficiency leads to pathogenic BP230 autoantibody and autoimmune bullous disease
Haeberle, Wei, Bieber, Goletz, Ludwig, Schmidt, Enk, Hadaschik
Journal of Allergy and Clinical Immunology.2018

Regulatory T cell deficient scurfy mice exhibit a Th2/M2-like inflammatory response in the skin
Haeberle*, Raker*, Haub, Kim, Weng, Yilmaz, Enk, Steinbrink, Schuppan, Hadaschik
J Dermatol Sci. 2017 Sep;87(3):309-311
Methods that will be used:
- molecular biology methods: immune precipitation, cloning, western blot, ELISA and antibody purification
- histological methods: preparation and staining of skin sections
- cell biology methods: cell culture of hybridoma cells, isolation of primary keratinocytes
- immunological methods: FACS, proliferations assays
- animal experiments
Cooperation partners:
University Hospital Lübeck, Dermatology
University Hospital Tübingen, Dermatology
University Hospital Mainz, Dermatology
Personal qualifications:
- Master of Science in Molecular Biology, Immunology, Cell Biology, Biochemistry
- Commitment to work with mice (FELESA course not mandatory)
- Enjoy working in the lab and in an international team
Keywords: