Ruprecht-Karls-Universität Heidelberg
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Albertelli, Alessandra

Alessandra Albertelli

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Supervisor:
Dr. Julien Béthune
Affiliation:
Biochemistry Center Heidelberg (BZH)

Field of interest:

Curriculum Vitae

2018 
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2018 
Fellowships
Fellowship "Torno Subito"

Title: "Use of imaging technique to define the 3D organization of the nucleus and role of non-coding RNA in chromatin organization”

Description: With this Fellowship, I had the chance to prove myself, writing a proposal concerning my scientific interest and goal. Trough the microscopy, I studied the role of the Nuclear pore complex during HIV infection, and the role of non-coding RNA involved in the 3D nucleus organization. From January to June 2018, I worked in Dr. Marina Lusic ́s laboratory (CIID, Heidelberg, Germany) and from September to November 2018, I worked in Prof. Irene Bozzoni ́s laboratory (“La Sapienza”, Rome, Italy)

2017 
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2017 
Internships
Title: “Characterisation of nuclear reorganization during lymphocyte differentiation”

Research Adviser: Prof. Massimiliano Pagani (INGM, Milan, Italy)

Description: I applied the skills developed during my Master Thesis to study the localisation of particular non-coding RNA in Regulatory T cell (Treg). I aimed to validate the data of the laboratory, setting a good protocol of RNA-FISH alone or in combination with immunofluorescence, and acquiring the fixed samples at confocal or wide-field microscopy.

2015 
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2017 
University education
Master Thesis in Molecular Biology

Title: “Role of linc-MAF-4 in human CD4+ Th1 lymphocytes”

Supervisor: Prof. Massimiliano Pagani (Universitá Statale di Milano, Milan, Italy)

Description: I aimed to study the importance of a long intergenic non-coding RNA, lincMAF4, during the CD4+ T cell differentiation.
lincMAF4 is Th1 cell-specific and represses the transcription of c-MAF, a transcription factor Th2 cell-specific, in Th1 cell line.
Our hypothesis: in Th1 cell line, lincMAF4 is responsible for the re-localization of c-MAF locus towards Lamin associated domain (LAD), trough the formation of a chromosome loop between lincMAF4 and the promoter of c-MAF .
Using confocal microscopy and imaging techniques (DNA-FISH, RNA-FISH, Immunofluorescence and a combination of these techniques), I could detect the linc-MAF-4 signal only in Th1 cell line, and I could visualize the co-localization of the c-MAF locus with LAD (in Th1 cell line) and with Nuclear Pore Complex (in Th2 cell line). I also performed RNA immunoprecipitation (RIP) experiment, to validate the interaction between lincMAF4 and LAD.

2014 
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2017 
University education
Master of Science in Medical Biotechnology and Molecular Medicine (oncology curriculum)

Universitá Statale di Milano (Milan, Italy)

2013 
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2014 
University education
Bachelor Thesis in Genetics

Universitá degli Studi della Tuscia (Viterbo, Italy)

Supervisor: Prof. Giorgio Prantera

Title: “Use of in vivo cytology to compare the phenotype between a chemical mutant strain of D. melanogaster and different interfered strains to determinate the genes responsible for the male-sterile phenotype”

Description: Identification of the genes responsible for the male-sterile phenotype in two different strains of D. melanogaster using RNA interference technique (RNAi) against potential candidates. We silenced single genes and used in vivo cytology to compare the phenotype of the fruit fly testis observed in mutated strains versus the phenotype of the RNAi manipulated fruit fly.

2010 
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2014 
University education
Bachelor of Science in Biotechnology (Industrial Curriculum)

Universitá degli Studi di Viterbo (Viterbo, Italy)

Publications

No publications found